eiMICRO
Our MissionEnable products that nourish, heal, and sustain people and planet
Triplebar proposes a future of abundant, accessible food and medicine. We believe biotechnology has the power to achieve this…but only if we can get from idea to product in a fraction of the time it takes today. We are building a tool to drive that future: eiMICRO.
eiMICRO: in silico organism design
Going from idea to strain to production is too hard, and it costs too much. Imagine if you could design a strain to make your product the same way you ask a question of ChatGPT? We are doing just that with eiMICRO – an in silico organism design SaaS – designed to make years of strain engineering a thing of the past
The Triplebar SolutionThe size and scale of our data powers BioAI, changing the way we design biology
We generate this data by vastly increasing the throughput of test. We miniaturize the unit of test in biology, the “test tube” into a picoliter volume microreactor. Through miniaturization, we can evaluate 10Ms microbes for high-volume protein production in a single day. We then index and sequence the top 0.1%, building vast data sets of phenotypically labeled genomes.
Screen 10M+ cells/day for Protein TiTer
AND sequence the 0.1% that are better
eimicro data generation Protein production screening Workflow
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Create Large Strain Library
Large diversity library containing rare events
- 10M Genomes/round
- 10 Mutations/genome
- 100M mutations = 10X oversample genome
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Create Microbioreactors
Cultivate cells in desired media, feed stock, pH, temperature to replicate scale Cells grow, express, and secrete protein
- 100,000,000 times smaller than shake flask
- 100,000 times smaller than microtiter plate
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Select the One in a Million
Using a specific secreted protein assay. Keep high performers, discard the rest
Sequencing of rare improvements generates large data sets that can be used to train models for faster improvement -
Confirm & Understand Improvements
Improvements are validated in microtiter plates and 2L fed-batch fermentation
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Repeat New Generations
Each generation allows us to run a cycle of evolution improving production round over round